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Lab protocols

Preparation of minimal media for uniform stable isotope (15N, 13C, 2H) labeling of protein

NMR sample preparation

Sample preparation for metabonomics of bio-fluids (Plasma/Serum, urine)

Data

Random coil chemical shifts for 1H, 13C, 15N nuclei in proteins

Lectures

Product operator of COSY and HSQC

NMR teaching web links

1. Basics of NMR by Joseph P. Hornak: Read the book

2. Lectures on NMR spectroscopy by James Keeler, University of Ottawa NMR facility. (Listen the lectures)

Lecture 1 : Energy levels  Lecture 2 : The vector model  Lecture 3   : Fourier Transformation 

Lecture 4   : Product Operators-1  Lecture 5   : Product Operators-2  Lecture 6  : Product Operators-3 

Lecture 7   : Two-Dimensional NMR-1  Lecture 8   : Two-Dimensional NMR-2  Lecture 9   : Relaxation-1

Lecture 10 : Relaxation-2  Lecture 11  : Relaxation-3  Lecture 12  : Coherence Selection-1

Lecture 13  : Coherence Selection-2 Lecture 14  : Coherence Selection-3